The Glyoxylic Reductase-Glycollic Oxidase System in Microorganism

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Oxidation and reduction of glycolic and glyoxylic acids in plants. II. Glyoxylic acid reductase.

Clagett, Tolbert, and Burris (1) discovered an enzyme of wide-spread occurrence in the green parts of many plants which catalyzes the oxidation of glycolic and L-lactic acids. The enzyme, which was partially purified from tobacco leaves, seemed to catalyze the oxidation of glycolic acid, through glyoxylic acid, to formic acid and CO, (2). In view of the possible importance of glycolic acid oxid...

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The kinetic properties of spinach leaf glyoxylic acid reductase.

The kinetic properties of spinach leaf glyoxylic acid reductase have been evaluated. In the presence of pyridine nucleotides, the enzyme catalyzes the reversible reduction of glyoxylate to glycolate and hydroxypyruvate to D( -)glycerate. The pH optima of the reductive reactions are between 6.0 and 6.5 ; the pH optima of the oxidative reactions are at 8.9. The enzyme is competitively inhibited b...

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Sulfhydryl studies of spinach leaf glyoxylic acid reductase.

Spinach leaf glyoxylic acid reductase, an enzyme containing two identical or similar subunits, has been shown to have 12 half-cystine residues. Four of these are necessary for catalytic function and can be titrated as free sulfhydryl groups. One mole of cysteinyl residues per mole of enzyme is essential to catalytic activity when evaluated under conditions favoring optimal activity. All free su...

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Mitochondrial preparations from the leaves of tobacco (Nicotiana tabacum). 433. Glycollic oxidase and fumarase activity.

Price, C. A. & Thimann, K. V. (1951). Arch. Biochem. Biophys. 33, 170. Remmert, L. F. & Lehninger, A. L. (1959). Proc. nat. Acad. Sci., Wash., 45, 1. Sacktor, B., O'Neill, J. J. & Cochran, D. G. (1958). J. biol. Chem. 233, 1233. Smillie, R. M. (1955). Aust. J. biol. Sci. 8, 186. Smillie, R. M. (1956). Aust. J. biol. Sci. 9, 81. Switzer, C. M. & Smith, F. G. (1957). Canad. J. Bot. 35, 515. Umbre...

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Microorganism Tracking Microscope System

Continuous observation of individual motile microorganisms is difficult, since their swimming speed is fast compared with their diameters. In fact, some bacteria can swim as fast as 50 diameters/s [1]. At this speed, microorganisms can quickly go out of the range of static measurement instruments, such as the field of view of optical microscopes. This problem can be solved by tracking the micro...

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ژورنال

عنوان ژورنال: Bulletin of the Agricultural Chemical Society of Japan

سال: 1959

ISSN: 0375-8397

DOI: 10.1080/03758397.1959.10857620